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. Author manuscript; available in PMC: 2007 Dec 5.
Published in final edited form as: Mutat Res. 2007 May 18;625(1-2):62–71. doi: 10.1016/j.mrfmmm.2007.05.004

Figure 4. Long-term treatment with WY-14,643 leads to progressive loss of histone H4K20me3 and H3K9me3 in the liver of wild type, but not Pparα-null mice.

Figure 4

Acid extracts of total histones prepared from livers of wild type (top panel) or Pparα-null (bottom panel) mice were separated by SDS-PAGE and subjected to immunoblotting using specific antibodies against histone H3K9me3 and histone H4K20me3 as detailed in Methods. Results (mean±S.D.) are presented as change in methylation in each treatment group (n=5) relative to control mice (all time points, n=15). Equal sample loading was confirmed by immunostaining against histone H3 and histone H4. Representative immunoblot images are shown. Asterisks (*) indicate significant difference from time/strain-matched control mice.