. Author manuscript; available in PMC: 2007 Dec 5.

Published in final edited form as: DNA Repair (Amst). 2006 Dec 26;6(3):355–366. doi: 10.1016/j.dnarep.2006.11.002

Table 1.

Kinetic parameters for dNTP incorporation opposite adenine or 2-OH-adenine by Kf^exo− (A) and Dpo4 (B) in a random and in a repeated sequence

A

Substrate	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (M⁻¹ s⁻¹ × 10⁵)	Selectivity^a

Kf^exo−
A
dTTP	0.0042(±0.0004)	0.6(±0.006)	1428(±14.8)	1
A^*
dTTP	0.21(±0.04)	0.43(±0.02)	20.5(±0.49)	69.7
dATP	18.1(±3.6)	0.049(±0.002)	0.03	47600
dCTP	27.7(±4.5)	0.19(±0.01)	0.67	2131
dGTP	2.1(±0.2)	0.141(±0.003)	0.7	2040
6A
dTTP	0.06(±0.007)	1.89(±0.04)	315(±3.68)	1
6A^*
dTTP	0.55(±0.11)	0.31(±0.01)	5.6(±0.1)	56.2
dATP	4.93(±0.97)	0.04(±0.002)	0.081(±0.002)	3888.9
dCTP	13.9(±1.7)	0.039(±0.001)	0.028	11250
dGTP	3.7(±0.7)	0.036(±0.001)	0.09(±0.002)	3500
B

Substrate	K_m (μM)	k_cat (s⁻¹)	k_cat/K_m (M⁻¹ s⁻¹ × 10⁴)	Selectivity^a

Dpo4
A
dTTP	0.79(±0.1)	0.035	4.43	1
A^*
dTTP	11.3(±1.7)	0.017(±0.001)	0.15	29.5
dATP	85.4(±13)	0.031(±0.003)	0.03	147.7
dCTP	69.2(±11)	0.038(±0.005)	0.05	88.6
dGTP	N.D.
6A
dTTP	8.8(±1.4)	0.11(±0.005)	1.25	1
6A^*
dTTP	32.9(±4.5)	0.073(±0.006)	0.22	5.7
dATP	41.7(±6.5)	0.023(±0.002)	0.05	25
dCTP	N.D.^b
dGTP	N.D.

Selectivity is defined as (k_cat/K_m)_TTP/(k_cat/K_m)_dXTP, where X is A, G or C. The value represents the number of correct (TTP) incorporation events for each incorrect (dXTP) incorporation.

Not detectable.