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. 2007 Dec;171(6):1733–1742. doi: 10.2353/ajpath.2007.070412

Figure 1.

Figure 1

High glucose and high insulin regulate eEF2 phosphorylation in MCT cells. Serum-deprived MCT cells were incubated with high glucose (30 mmol/L) (A), 5 mmol/L glucose + 25 mmol/L mannitol (B), or high insulin (1 nmol/L) (C) for the indicated period of time. Equal amounts of proteins were used for immunoblotting with Thr56 phospho-specific eEF2 antibody. Bottom panels show immunoblot analysis of the same samples with eEF2 antibody to assess loading. A representative blot from three to four independent experiments is shown and composite data are shown in histograms (‡P < 0.01, †P < 0.05 versus control by analysis of variance).