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. 2007 Dec;171(6):1733–1742. doi: 10.2353/ajpath.2007.070412

Figure 2.

Figure 2

High glucose and high insulin promote Ser366 phosphorylation of eEF2 kinase in MCT cells. Equal amounts of lysate proteins from cells incubated with high glucose (A) or high insulin (B) were immunoblotted with antibody against Ser366-phosphorylated eEF2 kinase. Bottom panels show assessment of loading by immunoblot analysis of the same samples with eEF2 kinase antibody. Composite data from three to four individual experiments are shown in histograms (‡P < 0.01, †P < 0.05 versus control).