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Immunofluorescent staining showing PMN sequestration (red, Alexa 568) and MPO presence (green, Alexa 488) in kidneys of WT (A–C) and Mpo−/− (E–G) mice subjected to I/R. Overlapping PMN and MPO data depict the fact that MPO is predominantly located around NIMP-R14-positive PMNs. D (WT) and H (Mpo−/−) show PMN infiltration in relation to structural changes. Shown are representative microphotographs of all groups. Original magnifications, ×200.
