Table 1.
Mass spectrometric and limited sequence analysis results
| Subunit | Peptide sequence(s) | m/z | Database match(es) | [MH+] (Δppm) | Homolog(s) |
|---|---|---|---|---|---|
| p160a | — | — | — | ||
| p160b | — | — | — | ||
| p110 | LVTTDLPPQLANLTVANGR | 1,993.052 | EST AA562494 | 1,993.108 (28) | Yeast Rgr1 and C. elegans Rgr1 |
| LLQLEILVEDKETGDGR | 1,928.064 | EST AA562494 | 1,928.035 (15) | ||
| ALVHSMQIDFIHQLVQSR* | 2,122.097 | EST AA562494 | 2,122.123 (12) | ||
| p96a | YNPPDHEVVAMAR | 1,498.672 | EST AA145180 | 1,498.711 (26) | Human Ring-3 and Drosophila fsh |
| NSNPDEIEIDFETLKPSTLR | 2,318.128 | GenBank X97573 | 2,318.152 (32) | ||
| p96b | VASDTQFYPGLGLALAFQDGSVHMVHR | 2,916.306 | EST AA022304 | 2,916.446 (48) | — |
| p78 | TKPGSPHWQSK | 1,252.641 | EST HSDHEI067†/ | 1,252.644 (2) | — |
| TIGR hgi THC173176† | |||||
| IEDPQIQAHWSNINDVYESSVK | 2,572.194 | EST W84147 | 2,572.232 (14) | — | |
| QVQEVGLDGTETYLQPLSMSQNLAR | 2,777.376 | EST AA313890† | 2,777.378 (1) | — | |
| p66 | EYRDAQEFGADVR | 1,555.674 | EST AA145180/ | 1,555.714 (26) | A possible degradation |
| EST AA403891 | product of p96a | ||||
| p55 | — | — | — | ||
| p36 | — | — | Yeast Med7 | ||
| p34 | MGGVSGMAGLGSTR | 1,280.626 | EST D76720 MUS 69B04 | 1,280.610 (12) | C. elegans ZK546.13 gene product |
| RPYPTDLEMR | 1,277.630 | EST AA432609 | 1,277.632 (2) | ||
| ISASNAVCAPLTWVPGDPR | 2,059.030 | EST AA432609 | 2,059.045 (7) | ||
| P33 | RPYPTDLEMR | 1,277.618 | EST AA432609 | 1,277.632 (10) | A possible degradation product of p34 |
| P32 | RKEEPSSIFQR | 1,376.681 | EST AA608236/ | 1,376.730 (35) | Yeast Med6 |
| GENBANK U78082† | |||||
| VLTAVHGIQSAFDEAMSYCR* | 2,303.141 | EST AA608236 | 2,303.097 (19) | ||
| QSPAQVIPLADYYIIAGVIYQAPDLGSVINSR | 3,431.779 | EST AA613346† | 3,431.822 (12) | ||
| p28a | YQYCDFLVK | 1,283.614 | EST AA085480† | 1,283.614 (0) | Drosophila TRF |
| HDAVYGPADTMIQYMELFNK | 2,343.074 | EST AA085480† | 2,343.079 (2) | Proximal protein | |
| p28b | NFEAQLKPLVHLEK | 1,665.867 | W242958†/ | 1,665.934 (40) | — |
| AA461545†/ | |||||
| AA262939† | |||||
| p21 | — | — | Yeast Srb7 |
m/z, experimental monoisotopic mass. [MH+], calculated theoretical mass plus one proton. Δppm, difference between measured and theoretical mass, in parts per million.
*
Identified only by mass spectrometry.
†
Human ESTs.