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. 2007 Dec;171(6):1800–1810. doi: 10.2353/ajpath.2007.070261

Figure 5.

Figure 5

Co-localization of RA175, Par-3, Jam-C, and Nectin-3 in the elongating spermatids (step 9 to 12) of the Ra175+/+ testis and loss of their co-localization in the Ra175−/− testis. A–C, G–I, M–O, S–U: Ra175+/+; D–F, J–L, P–R, V–X: Ra175−/−. A–C: Anti-RA175C; D–F, G–I, J–L: anti-Jam-C; M–O, P–R: anti-Par-3; and S–X: anti-Nectin-3. A, D, G, J, M, P, S, V: Images of spermatids stained with antibodies (red) and Hoechst staining (blue). B, E, H, K, N, Q, T, W: Images of spermatids stained with FITC-conjugated PNA (green), and Hoechst staining (blue). C, F, I, L, O, R, U, X: Merged images of spermatids stained with antibody (red), FITC-conjugated PNA (green), and Hoechst staining (blue). Jam-C and Par-3 immunoreactivities (D and P) were not detected in the dorsal region of the head of elongating spermatids except for some abnormal localization of Jam-C (J). Scale bar = 5 μm.