Figure 9.

The effect of HA on cell-associated versican and perlecan expression. a: MCs were incubated in the presence or absence of 5 mg/ml of HA and then fixed and immunostained with anti-versican or anti-perlecan antibodies and visualized with anti-rabbit FITC-conjugated IgG. b: MCs were radiolabeled for 24 hours with [³⁵S]sulfate in the presence (filled circles) or absence (open circles) of 5 mg/ml of HA. Cell layer proteoglycans were then quantified using DEAE ion exchange and gel exclusion chromatography as described in Materials and Methods. The results are expressed as ³⁵S-proteoglycans (cpm/10³ cells). Data shown are representative of three independent experiments.