Table 1.

Total Proteoglycan Synthesis by MC

Conditions	0.2% LH	IL-1β	PDGF-BB	20% FCS
Hyaluronan*	5 ± 1	65 ± 24§	129 ± 11§	161 ± 18§
CSPG†	19 ± 5	45 ± 6‡	123 ± 37§	167 ± 48§
DSPG†	351 ± 74	416 ± 138	807 ± 242‡	675 ± 178‡
HSPG†	167 ± 45	215 ± 31	239 ± 36	255 ± 76
Cell numbers (×10−3)	41 ± 4	43 ± 15	66 ± 17	65 ± 6

Cells were metabolically labeled with [³⁵S]-sulfate or [³H]-glucosamine and incubated with the agents shown for 36 hours, as described in Materials and Methods. The conditioned medium and cell extracts were then prepared and specific activity counted as described. 

^*[³H]-glucosamine dpm/10³ cells ± 1 SD (n = 3). 

^†[³⁵S]-sulfate dpm/10³ cells ± 1 SD (n = 3). 

Statistical difference to control levels is shown as 

^‡P < 0.05 and 

^§P < 0.01.