Figure 3.

Immunophenotype of intrahepatic bile ducts of the PCK rat. Immunostaining using liver sections for epithelial markers (CK19, E-cadherin) and mesenchymal markers (vimentin, fibronectin) was performed as described in Materials and Methods. Immunophenotype of C-type bile ducts of the PCK rats and interlobular bile ducts of the adult normal rats (arrows) was identical. In F-type bile ducts of the PCK rats, reduction of the expression of biliary epithelial marker CK19 was observed, whereas E-cadherin expression was unchanged. F-type showed immunoreactivity for mesenchymal markers, vimentin and fibronectin. Original magnifications, ×1000.