Table 1.

Sequences of the Primers and PCR Conditions Used in This Study

Gene	Sequences	Annealing temperature (°C)	PCR cycles
CK19	5′-GTACCAGAAGCAGGGACCCG-3′	60	22
	5′-TTCCAGGGCAGCTTTCATGC-3′
E-cadherin	5′-GAGGGTTTTCACTGGTTGTT-3′	55	28
	5′-GTAAAGACACTCTGGAGGTG-3′
Vimentin	5′-TTCAAGAACACCCGCACCAAC-3′	55	29
	5′-GCCTTCCAGCAGCTTCCTGTA-3′
Procollagen type I	5′-CATAAAGGGTCATCGTGGCTTC-3′	65	35
	5′-GTGATAGGTGATGTTCTGGGAG-3′
Fibronectin	5′-CCTACAACATCATAGTGGAG-3′	55	35
	5′-CTTGTAGTTGACACCGTTGT-3′
α-SMA	5′-CATCCACGAAACCACCTATA-3′	55	40
	5′-CTGTTTGCTGATCCACATCT-3′
TGF-β1	5′-CAATTCCTGGCGTTACCTTG-3′	60	35
	5′-GAAGCAGTAGTTGGTATCCA-3′
TβR-II	5′-GTGAAGAACGATTTGACCTG-3′	50	40
	5′-GTTGTCTTTCATGCTCTCCA-3′
β-Actin	5′-ACCTTCAACACCCCAGCCATGTACG-3′	60	25
	5′-CTGATCCACATCTGCTGGAAGGTGG-3′

CK, cytokeratin; SMA, smooth muscle actin; TGF, transforming growth factor; TβR-II, TGF-β type II receptor.