Figure 5.

Regulation of GFAP transcription by EGb 761. U373 cells (A, open bar) and murine primary astrocytes (A, closed bar) were transfected with the GFAP promoter-driven luciferase reporter plasmid, or U373 cells were transfected with AP1-dependent luciferase reporter plasmid (C, open bar) or NF-κB-dependent luciferase reporter plasmid (C, closed bar) using Lipofectamine. Transfected cells were treated with EGb 761 at concentrations as indicated for 2 days, and harvested for luciferase activity assay. Untransfected U373 cells were also treated with EGb 761 at concentrations as indicated for 2 days and harvested for whole cell lysates for electrophoretic mobility shift assay (B) in the presence of ³²P-labeled AP1, AP2, NF-κB, and CREB binding site-containing oligonucleotides. Open arrow: unbound probe; closed arrow: protein-probe complex. FP: free ³²P-labeled probe (no cell lysates added).