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. 2007 Dec;171(6):1952–1965. doi: 10.2353/ajpath.2007.070017

Figure 4.

Figure 4

Protein extracts of colon prepared from four SIV-infected macaques with chronic diarrhea (lanes 2 to 5, 10 and 11, 14 and 15), three non-SIV-infected macaques with diarrhea (lanes 6 to 8, 12 and 13, 16 and 17) and one control macaque (lane 9) were incubated with biotin-labeled wild-type STAT3-binding oligonucleotide sequence (lanes 2 to 9) or mutant (lanes 10 to 13) or streptavidin-conjugated agarose beads only (lanes 14 to 17). The protein-DNA complexes were recovered as described in the Materials and Methods and analyzed by immunoblotting using antibodies against p-STAT3 (A) and t-STAT3 (B). Thus, A shows the presence or absence of p-STAT3 and B shows the presence of t-STAT3 (p-STAT3 and np-STAT3) in the same sample shown in A.