Figure 3.

The detection of insoluble αS forms by ICC requires the removal of soluble αS forms. a: Stably αS-transfected MES cells were conditioned in serum-free medium supplemented with the PUFA 18:3 (right) or without (left) for 16 hours and processed for ICC with anti-αS Ab (LB509, Zymed) followed by Alexa 488 (green). b: Cells were treated and processed for ICC as in a, but the soluble αS forms were washed out by prepermeabilization of the cells with 0.002 Triton X-100 before fixation. Note that the pictures in a and b were taken under identical conditions of laser intensity and exposure.