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. 2007 Dec;171(6):2000–2011. doi: 10.2353/ajpath.2007.070373

Figure 8.

Figure 8

PUFA-induced αS oligomers induce cytotoxicity. αS-Overexpressing MES dopaminergic cells were conditioned with BSA alone or BSA plus FA (125 μmol/L) for the indicated time. a: Samples (15 μg) of high-speed supernatant (after 280,000 × g) were incubated at 65°C overnight before gel loading and blotting with H3C Ab. B, BSA-treated; 2, 8, 16, 24, 48 = hours of conditioning in the presence of 18:3. b: Densitometric analysis of the blot in a, presented as the ratio of total oligomers to monomer shown in comparison to the number of inclusions in sister cultures treated in parallel for each time point. Inclusions were counted as described in legend of Figure 6b. nd, not done. c: Values of XTT assay representing cellular metabolic activity at the indicated time points in sister cultures (conducted in parallel to a and b), presented as the relative activity in 18:3-treated to 18:1 (control) treated cultures. Mean of five to six replicates at each time point ± SD; experiment repeated three times.