Table 1 Polymerase chain reaction primers, restriction enzymes, and separation conditions for genotyping.
| Gene (polymorphism) | Primer sequence | Restriction enzyme (Temperature) | Separation conditions |
|---|---|---|---|
| SOD1 (A→C, +35 exon3/intron3) | Forward: 5′CTATCCAGAAAACACGGTGGGCC3′Reverse: 5′TCTATATTCAATCAAATGCTACAAAACC3′ | Hha I (37°C) | 2% agarose |
| SOD2 (Val16Ala, T→C) | Forward: 5′CGCAGCCCAGCCGTGCGTA3′Reverse: 5′GTGAGGTTCCAGGGCGCCGT3′ | Bsa WI (60°C) | 2% agarose |
| SOD3 (Arg213Gly, A→G) | Forward: 5′GCAACCAGGCCAGCGTGGAGAACGGGAA3′Reverse: 5′CCAGAGGAGAAGCTCAAAGGCAGA3′ | Mwo I (60°C) | 3% MS agarose |
| Catalase (A→T, promoter) | Forward: 5′AATCAGAAGGCAGTCCTCCC3′Reverse: 5′TCGGGGAGCACAGAGTGTAC3′ | Hinf I (37°C) | 2% agarose |