Table 1.

Contour length of ParR bound parC-containing fragments

parC DNA used	Fragment size	Measured length of ParR-bound fragment	Molecules measured, n
Supercoiled parC	466	335  ±  38	266
Linear parC	466	456  ±  22	287
Supercoiled parC22	439	419  ±  26	170
Supercoiled parC33	390	371  ±  25	164

ParR (100 ng) was bound to 100 ng pMD330 DNA (wt parC), pAB1903 DNA (parC22), or pMD333 DNA (parC33) and prepared for electron microscopy. The contour lengths in bp (±SD) of free and protein-bound DNA fragments were measured and analysed statistically.