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Temperature dependence of the interaction of the fully complementary oligonucleotide 430 (□) with the matrix-bound PNA (200 nM) compared with single mismatch probes: mm9 (○), mm2 (▾), mm12 (•), mm8 (♦), and mm4 (■). The relative migration, in terms of data points collected to the midpoint of the detected peak, was determined with respect to the noninteracting oligonucleotide R.
