Table 2.

Thermodynamic parameters for DNA–PNA duplex formation at 37°C

DNA	Mismatch position and type	ΔS°, eu	ΔH°, kcal/mol	ΔG°(37°C), kcal/mol	Kdiss (37°C), M
430	None	−164	−64	−14.7	3.1  × 10−10
mm1	1 (G:G)	−122	−50	−11.9	4.2  × 10−9
mmgt	1 (G:T)	−135	−54	−12.1	3.2  × 10−9
mm2	2 (T:T)	−129	−51	−11.2	1.3  × 10−8
mm3	3 (G:G)	−179	−64	−8.8	6.3  × 10−7
mm4	4 (G:G)	−213	−74	−8.0	2.3  × 10−6
mm5	5 (T:T)	−142	−55	−11.5	8.4  × 10−9
mm6	6 (T:T)	−138	−54	−11.2	1.2  × 10−8
mm13	6 (C:T)	−121	−47	−10.8	1.0  × 10−7
mm7	7 (A:A)	−261	−90	−9.9	1.0  × 10−7
mm12	7 (G:A)	−169	−62	−9.9	1.0  × 10−7
mm8	8 (C:C)	−88	−37	−9.6	1.6  × 10−7
mm9	9 (T:T)	−123	−50	−11.8	4.8  × 10−9
mm10	10 (A:A)	−103	−43	−11.3	1.1  × 10−8
mm11	11 (T:T)	−138	−55	−12.6	1.3  × 10−9
429	7 (A:A), 11 (G:T)	−130	−50	−9.5	2.3  × 10−7

eu, cal K⁻¹·mol⁻¹.