Abstract
A sequence-specific modification methylase (M . SinI) was isolated and purified from Escherichia coli harboring a derivative of recombinant plasmid pSI4 (see accompanying manuscript: C. Karreman and A. de Waard, J. Bacteriol. 170:2527-2532, 1988), which contains a Salmonella infantis DNA insert. The enzyme uniquely methylates the internal deoxycytidylate residue in the nucleotide sequence GG(A/T)MeCC, thereby protecting DNA completely against cleavage by restriction endonuclease R . SinI or R . AvaII [GG(A/T)CC], and in part against cleavage by R . Sau96I (GGNCC).
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Butkus V., Klimasauskas S., Kersulyte D., Vaitkevicius D., Lebionka A., Janulaitis A. Investigation of restriction-modification enzymes from M. varians RFL19 with a new type of specificity toward modification of substrate. Nucleic Acids Res. 1985 Aug 26;13(16):5727–5746. doi: 10.1093/nar/13.16.5727. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Casadaban M. J., Cohen S. N. Analysis of gene control signals by DNA fusion and cloning in Escherichia coli. J Mol Biol. 1980 Apr;138(2):179–207. doi: 10.1016/0022-2836(80)90283-1. [DOI] [PubMed] [Google Scholar]
- Devereux J., Haeberli P., Smithies O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res. 1984 Jan 11;12(1 Pt 1):387–395. doi: 10.1093/nar/12.1part1.387. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jentsch S., Günthert U., Trautner T. A. DNA methyltransferases affecting the sequence 5'CCGG. Nucleic Acids Res. 1981 Jun 25;9(12):2753–2759. doi: 10.1093/nar/9.12.2753. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karreman C., Tandeau de Marsac N., de Waard A. Isolation of a deoxycytidylate methyl transferase capable of protecting DNA uniquely against cleavage by endonuclease R.Aqu I (isoschizomer of Ava I). Nucleic Acids Res. 1986 Jul 11;14(13):5199–5205. doi: 10.1093/nar/14.13.5199. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Karreman C., de Waard A. Cloning and complete nucleotide sequences of the type II restriction-modification genes of Salmonella infantis. J Bacteriol. 1988 Jun;170(6):2527–2532. doi: 10.1128/jb.170.6.2527-2532.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lupker H. S., Dekker B. M. Purification of the sequence-specific endonuclease SinI from Salmonella infantis. Biochim Biophys Acta. 1981 Jul 27;654(2):297–299. doi: 10.1016/0005-2787(81)90185-4. [DOI] [PubMed] [Google Scholar]
- Matvienko N. I., Kramarov V. M., Pachkunov D. M. Isolation and some properties of the site-specific endonuclease and methylase Bme2161 from Bacillus megaterium 216. Eur J Biochem. 1987 Jun 15;165(3):565–570. doi: 10.1111/j.1432-1033.1987.tb11477.x. [DOI] [PubMed] [Google Scholar]
- Maxam A. M., Gilbert W. Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980;65(1):499–560. doi: 10.1016/s0076-6879(80)65059-9. [DOI] [PubMed] [Google Scholar]
- Ohmori H., Tomizawa J. I., Maxam A. M. Detection of 5-methylcytosine in DNA sequences. Nucleic Acids Res. 1978 May;5(5):1479–1485. doi: 10.1093/nar/5.5.1479. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sussenbach J. S., Steenbergh P. H., Rost J. A., van Leeuwen W. J., van Embden J. D. A second site-specific restriction endonuclease from Staphylococcus aureus. Nucleic Acids Res. 1978 Apr;5(4):1153–1163. doi: 10.1093/nar/5.4.1153. [DOI] [PMC free article] [PubMed] [Google Scholar]