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. 1998 Jul 21;95(15):8574–8579. doi: 10.1073/pnas.95.15.8574

Figure 4.

Figure 4

E2F–DNA binding activity in the cytokine-dependent 6–1 cells. (A) Identification of E2F species. Whole-cell extracts prepared from growth-arrested (Left) or asynchronously growing (Right) 6–1 cells were subjected to an E2F gel shift assay. (B) Effect of p130 overexpression on E2F-DNA binding activity. Whole cell lysates were prepared from cells overexpressing p130 and were subjected to an E2F gel shift assay. Anti-p130 used in the assay cross-reacted with p107. (C) Effect of pRBΔS/T-P overexpression on E2F–DNA binding activity. Whole-cell lysate was prepared from the asynchronously growing cells overexpressing pRBΔS/T-P and were subjected to an E2F gel shift assay. Wild-type (wt) or mutant (mt) E2F gel shift oligonucleotides were used as competitors (A–C). The positions of free E2F and E2Fs complexed with pRB family proteins are indicated (A–C).