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. 1981 Jan 1;88(1):234–240. doi: 10.1083/jcb.88.1.234

Direct visualization of fluorescein-labeled microtubules in vitro and in microinjected fibroblasts

PMCID: PMC2111708  PMID: 7193677

Abstract

Microtubule proteins and tubulin have been purified from brain and labeled with dichlorotriazinyl fluorescein (DTAF). This procedure compromises neither the polymerizability of the proteins nor their affinities for unlabeled proteins. Within 15 min after microinjection of either DTAF-microtubule proteins or DTAF-tubulin into cultured gerbil fibroma cells, there was an evolution of a fluorescent fibrillar pattern with a distribution similar to that of the microtubular network seen after staining with fluorescent antitubulin. These filaments were colchicine sensitive and could be seen to elongate with time. DTAF- labeled microtubule accessory proteins from brain were not incorporated into filaments and appeared to label autophagic vacuoles.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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