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Example of the experimental procedure. (a) Autocorrelation curves for beacon (○) and control (•) at T = 45°C, 0.2 M NaCl. Both beacon and control have loops of 21 T residue. (b) Ratio of the two curves shown in a. The line is a three-parameter exponential fit to the data (see text) giving τreaction = 24.2 ± 0.6 μs.
