Abstract
To investigate potential heterogeneity and developmental changes in basement membranes during embryogenesis, we performed immunohistochemical analyses on lens capsules in chicken embryos of different ages using domain-specific monoclonal antibodies against type IV collagen. We found that the capsule of the newly formed lens stained uniformly with antibodies against this component of basement membranes, but with increasing age and differentiation of the lens cells the anterior lens capsule remained brightly fluorescent while staining of the posterior capsule became relatively much less intense. This antero- posterior gradient of anti-type IV collagen antibody reactivity demonstrated that developmentally-regulated changes can occur within a single, continuous basement membrane.
Full Text
The Full Text of this article is available as a PDF (1.2 MB).
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bard J. B., Hay E. D. The behavior of fibroblasts from the developing avian cornea. Morphology and movement in situ and in vitro. J Cell Biol. 1975 Nov;67(2PT1):400–418. doi: 10.1083/jcb.67.2.400. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Bernfield M. R., Banerjee S. D., Cohn R. H. Dependence of salivary epithelial morphology and branching morphogenesis upon acid mucopolysaccharide-protein (proteoglycan) at the epithelial surface. J Cell Biol. 1972 Mar;52(3):674–689. doi: 10.1083/jcb.52.3.674. [DOI] [PMC free article] [PubMed] [Google Scholar]
- COULOMBRE A. J., COULOMBRE J. L. The role of intraocular pressure in the development of the chick eye. IV. Corneal curvature. AMA Arch Ophthalmol. 1958 Apr;59(4):502–506. doi: 10.1001/archopht.1958.00940050058005. [DOI] [PubMed] [Google Scholar]
- COULOMBRE A. J. The role of intraocular pressure in the development of the chick eye. II. Control of corneal size. AMA Arch Ophthalmol. 1957 Feb;57(2):250–253. doi: 10.1001/archopht.1957.00930050260015. [DOI] [PubMed] [Google Scholar]
- COULOMBRE J. L., COULOMBRE A. J. LENS DEVELOPMENT: FIBER ELONGATION AND LENS ORIENTATION. Science. 1963 Dec 13;142(3598):1489–1490. doi: 10.1126/science.142.3598.1489. [DOI] [PubMed] [Google Scholar]
- Coulombre A. J., Herrmann H. Lens development. 3. Relationship between the growth of the lens and the growth of the outer eye coat. Exp Eye Res. 1965 Dec;4(4):302–311. doi: 10.1016/s0014-4835(65)80045-8. [DOI] [PubMed] [Google Scholar]
- Ekblom P., Alitalo K., Vaheri A., Timpl R., Saxén L. Induction of a basement membrane glycoprotein in embryonic kidney: possible role of laminin in morphogenesis. Proc Natl Acad Sci U S A. 1980 Jan;77(1):485–489. doi: 10.1073/pnas.77.1.485. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitch J. M., Gibney E., Sanderson R. D., Mayne R., Linsenmayer T. F. Domain and basement membrane specificity of a monoclonal antibody against chicken type IV collagen. J Cell Biol. 1982 Nov;95(2 Pt 1):641–647. doi: 10.1083/jcb.95.2.641. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fitch J. M., Linsenmayer T. F. Monoclonal antibody analysis of ocular basement membranes during development. Dev Biol. 1983 Jan;95(1):137–153. doi: 10.1016/0012-1606(83)90013-1. [DOI] [PubMed] [Google Scholar]
- Linsenmayer T. F., Fitch J. M., Schmid T. M., Zak N. B., Gibney E., Sanderson R. D., Mayne R. Monoclonal antibodies against chicken type V collagen: production, specificity, and use for immunocytochemical localization in embryonic cornea and other organs. J Cell Biol. 1983 Jan;96(1):124–132. doi: 10.1083/jcb.96.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mayne R., Sanderson R. D., Wiedemann H., Fitch J. M., Linsenmayer T. F. The use of monoclonal antibodies to fragments of chicken type IV collagen in structural and localization studies. J Biol Chem. 1983 May 10;258(9):5794–5797. [PubMed] [Google Scholar]
- Mayne R., Wiedemann H., Dessau W., Von der Mark K., Bruckner P. Structural and immunological characterization of type IV collagen isolated from chicken tissues. Eur J Biochem. 1982 Aug;126(2):417–423. doi: 10.1111/j.1432-1033.1982.tb06796.x. [DOI] [PubMed] [Google Scholar]
- Mayne R., Zettergren J. G. Type IV collagen from chicken muscular tissues. Isolation and characterization of the pepsin-resistant fragments. Biochemistry. 1980 Aug 19;19(17):4065–4072. doi: 10.1021/bi00558a025. [DOI] [PubMed] [Google Scholar]
- Ostrer H., Beebe D. C., Piatigorsky J. Beta-crystallin mRNAs: differential distribution in the developing chicken lens. Dev Biol. 1981 Sep;86(2):403–408. doi: 10.1016/0012-1606(81)90198-6. [DOI] [PubMed] [Google Scholar]
- Piatigorsky J. Lens differentiation in vertebrates. A review of cellular and molecular features. Differentiation. 1981;19(3):134–153. doi: 10.1111/j.1432-0436.1981.tb01141.x. [DOI] [PubMed] [Google Scholar]
- Vracko R. Basal lamina scaffold-anatomy and significance for maintenance of orderly tissue structure. Am J Pathol. 1974 Nov;77(2):314–346. [PMC free article] [PubMed] [Google Scholar]
- Wakely J., England M. A. Scanning electron microscopical and histochemical study of the structure and function of basement membranes in the early chick embryo. Proc R Soc Lond B Biol Sci. 1979 Dec 31;206(1164):329–352. doi: 10.1098/rspb.1979.0109. [DOI] [PubMed] [Google Scholar]
- Wolpow E. R., Marimont R. B. Mathematical approach to measurements on embryonic chick lens. J Morphol. 1967 Jan;121(1):71–80. doi: 10.1002/jmor.1051210107. [DOI] [PubMed] [Google Scholar]
- Young R. W., Ocumpaugh D. E. Autoradiographic studies on the growth and development of the lens capsule in the rat. Invest Ophthalmol. 1966 Dec;5(6):583–589. [PubMed] [Google Scholar]