Figure 8.

The globular tail domain of myosin-Va is targeted to the centrosome in mammalian cells. (A) Immunofluorescence staining for myosin-V in interphase S91 cells using the antitail antibody. (B–E) Live, transfected cells imaged by fluorescence microscopy using a filter set for fluorescein isothiocyanate (B and D) and phase-contrast microscopy (C) or differential interference contrast microscopy (E) of the same fields, respectively. (B and C) Recently divided S91 cells transfected with the GFP–globular tail construct. (D and E) B16-F10 cells transfected with the pS65T-C1 control vector showing diffuse fluorescence from GFP alone.