Figure 3.

Experimental validation of NF-κB motif modules with age. (A) NF-κB-containing motif modules are experimentally responsive to altered NF-κB activity. Each row represents a motif module composed of the indicated genes (the last row represents the overlap of all age-associated genes); each column represents an experiment. Each square is the average expression value of genes in the indicated motif module. NF-κB modules are specifically induced by p50 and p65 expression in primary human keratinocytes (left) and repressed by IκBα expression in primary human fibroblasts (right). (*) P < 0.05 between experimental treatment and control, Student’s t-test. (B) NF-κB DNA-binding activity increase with age in mouse tissue. Shown is the relative level of NF-κB DNA-binding activity in the indicated organ of young (Y; 1 mo) and old (O; 22 mo) wild-type mice. Orange line indicates the average activity. (*) P < 0.005 between young and old for each organ, Student’s t-test.