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The majority of centromeres show detachment only once during S phase. CEN15-lacOs GFP-LacI GFP-TUB1 cells (T5280) were treated with α-factor and subsequently released to fresh medium. After 20 min, GFP images were collected every 20 sec for 40 min. The colored lines show the percentage of cells with the following cumulative number of CEN15 detachment from microtubules: zero (green), once (red,) and twice or more (blue). The black line shows the percentage of cells with buds.
