Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Aug;170(8):3738–3741. doi: 10.1128/jb.170.8.3738-3741.1988

Two unusual pilin sequences from different isolates of Pseudomonas aeruginosa.

B L Pasloske 1, P A Sastry 1, B B Finlay 1, W Paranchych 1
PMCID: PMC211353  PMID: 2841299

Abstract

The pilin genes of two Pseudomonas aeruginosa strains isolated from two different patients with cystic fibrosis were cloned and sequenced. The predicted protein sequences of these two pilins had several unusual features compared with other published P. aeruginosa pilin sequences.

Full text

PDF
3738

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson B. J., Mattick J. S., Cox P. T., Kristo C. L., Egerton J. R. Western blot (immunoblot) analysis of the fimbrial antigens of Bacteroides nodosus. J Bacteriol. 1987 Sep;169(9):4018–4023. doi: 10.1128/jb.169.9.4018-4023.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bacon D. J., Anderson W. F. Multiple sequence alignment. J Mol Biol. 1986 Sep 20;191(2):153–161. doi: 10.1016/0022-2836(86)90252-4. [DOI] [PubMed] [Google Scholar]
  3. Bodey G. P., Bolivar R., Fainstein V., Jadeja L. Infections caused by Pseudomonas aeruginosa. Rev Infect Dis. 1983 Mar-Apr;5(2):279–313. doi: 10.1093/clinids/5.2.279. [DOI] [PubMed] [Google Scholar]
  4. Dalrymple B., Mattick J. S. An analysis of the organization and evolution of type 4 fimbrial (MePhe) subunit proteins. J Mol Evol. 1987;25(3):261–269. doi: 10.1007/BF02100020. [DOI] [PubMed] [Google Scholar]
  5. Dixon R. The xylABC promoter from the Pseudomonas putida TOL plasmid is activated by nitrogen regulatory genes in Escherichia coli. Mol Gen Genet. 1986 Apr;203(1):129–136. doi: 10.1007/BF00330393. [DOI] [PubMed] [Google Scholar]
  6. Frost L. S., Carpenter M., Paranchych W. N-methylphenylalanine at the N-terminus of pilin isolated from Pseudomonas aeruginosa K. Nature. 1978 Jan 5;271(5640):87–89. doi: 10.1038/271087a0. [DOI] [PubMed] [Google Scholar]
  7. Gussin G. N., Ronson C. W., Ausubel F. M. Regulation of nitrogen fixation genes. Annu Rev Genet. 1986;20:567–591. doi: 10.1146/annurev.ge.20.120186.003031. [DOI] [PubMed] [Google Scholar]
  8. Hermodson M. A., Chen K. C., Buchanan T. M. Neisseria pili proteins: amino-terminal amino acid sequences and identification of an unusual amino acid. Biochemistry. 1978 Feb 7;17(3):442–445. doi: 10.1021/bi00596a010. [DOI] [PubMed] [Google Scholar]
  9. Janssen D. B., Herst P. M., Joosten H. M., van der Drift C. Nitrogen control in Pseudomonas aeruginosa: a role for glutamine in the regulations of the synthesis of nadp-dependent glutamate dehydrogenase, urease and histidase. Arch Microbiol. 1981 Feb;128(4):398–402. doi: 10.1007/BF00405920. [DOI] [PubMed] [Google Scholar]
  10. Johnson K., Parker M. L., Lory S. Nucleotide sequence and transcriptional initiation site of two Pseudomonas aeruginosa pilin genes. J Biol Chem. 1986 Nov 25;261(33):15703–15708. [PubMed] [Google Scholar]
  11. Mattick J. S., Bills M. M., Anderson B. J., Dalrymple B., Mott M. R., Egerton J. R. Morphogenetic expression of Bacteroides nodosus fimbriae in Pseudomonas aeruginosa. J Bacteriol. 1987 Jan;169(1):33–41. doi: 10.1128/jb.169.1.33-41.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. McKern N. M., O'Donnell I. J., Inglis A. S., Stewart D. J., Clark B. L. Amino acid sequence of pilin from Bacteroides nodosus (strain 198), the causative organism of ovine footrot. FEBS Lett. 1983 Nov 28;164(1):149–153. doi: 10.1016/0014-5793(83)80039-8. [DOI] [PubMed] [Google Scholar]
  13. Minton N. P., Clarke L. E. Identification of the promoter of the Pseudomonas gene coding for carboxypeptidase G2. J Mol Appl Genet. 1985;3(1):26–35. [PubMed] [Google Scholar]
  14. Moore L. J., Rutter J. M. Antigenic analysis of fimbrial proteins from Moraxella bovis. J Clin Microbiol. 1987 Nov;25(11):2063–2070. doi: 10.1128/jcm.25.11.2063-2070.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Paranchych W., Frost L. S. The physiology and biochemistry of pili. Adv Microb Physiol. 1988;29:53–114. doi: 10.1016/s0065-2911(08)60346-x. [DOI] [PubMed] [Google Scholar]
  16. Paranchych W., Sastry P. A., Volpel K., Loh B. A., Speert D. P. Fimbriae (pili): molecular basis of Pseudomonas aeruginosa adherence. Clin Invest Med. 1986;9(2):113–118. [PubMed] [Google Scholar]
  17. Parker J. M., Guo D., Hodges R. S. New hydrophilicity scale derived from high-performance liquid chromatography peptide retention data: correlation of predicted surface residues with antigenicity and X-ray-derived accessible sites. Biochemistry. 1986 Sep 23;25(19):5425–5432. doi: 10.1021/bi00367a013. [DOI] [PubMed] [Google Scholar]
  18. Pasloske B. L., Finlay B. B., Paranchych W. Cloning and sequencing of the Pseudomonas aeruginosa PAK pilin gene. FEBS Lett. 1985 Apr 22;183(2):408–412. doi: 10.1016/0014-5793(85)80821-8. [DOI] [PubMed] [Google Scholar]
  19. Pasloske B. L., Joffe A. M., Sun Q., Volpel K., Paranchych W., Eftekhar F., Speert D. P. Serial isolates of Pseudomonas aeruginosa from a cystic fibrosis patient have identical pilin sequences. Infect Immun. 1988 Mar;56(3):665–672. doi: 10.1128/iai.56.3.665-672.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Sastry P. A., Finlay B. B., Pasloske B. L., Paranchych W., Pearlstone J. R., Smillie L. B. Comparative studies of the amino acid and nucleotide sequences of pilin derived from Pseudomonas aeruginosa PAK and PAO. J Bacteriol. 1985 Nov;164(2):571–577. doi: 10.1128/jb.164.2.571-577.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sastry P. A., Pearlstone J. R., Smillie L. B., Paranchych W. Studies on the primary structure and antigenic determinants of pilin isolated from Pseudomonas aeruginosa K. Can J Biochem Cell Biol. 1985 Apr;63(4):284–291. doi: 10.1139/o85-042. [DOI] [PubMed] [Google Scholar]
  22. Speert D. P., Eftekhar F., Puterman M. L. Nonopsonic phagocytosis of strains of Pseudomonas aeruginosa from cystic fibrosis patients. Infect Immun. 1984 Mar;43(3):1006–1011. doi: 10.1128/iai.43.3.1006-1011.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Watts T. H., Kay C. M., Paranchych W. Spectral properties of three quaternary arrangements of Pseudomonas pilin. Biochemistry. 1983 Jul 19;22(15):3640–3646. doi: 10.1021/bi00284a016. [DOI] [PubMed] [Google Scholar]
  24. Woods D. E., Straus D. C., Johanson W. G., Jr, Berry V. K., Bass J. A. Role of pili in adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Infect Immun. 1980 Sep;29(3):1146–1151. doi: 10.1128/iai.29.3.1146-1151.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. Yanisch-Perron C., Vieira J., Messing J. Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene. 1985;33(1):103–119. doi: 10.1016/0378-1119(85)90120-9. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES