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. 1998 Jul 21;95(15):8686–8691. doi: 10.1073/pnas.95.15.8686

Figure 1.

Figure 1

Targeted disruption of the Mttp gene. (A) A map of the Mttp locus, together with the sequence-replacement gene-targeting vector and the targeted Mttp allele. Exons (open boxes), introns (thin line), and start of the MTP ORF (arrow) are indicated. The gene-targeting event replaced exon 1 coding sequences with a cassette containing a neomycin-resistance gene (neo) driven by a phosphoglycerate kinase 1 promoter and a green fluorescent protein cDNA fragment (solid box). Analysis of gene-targeting events and mouse genotyping was performed by Southern blot analysis of SacI-digested genomic DNA, using a 1.3-kb probe (thick bar) extending from exon 2 to exon 3. The probe, which was labeled by random priming, was generated by PCR with oligonucleotides 5′-TGAGCGGCTATACAAGCTCAC-3′ and 5′-CTGGAAGATGCTCTTCTCGC-3′. (B) Southern blot of SacI-digested DNA from E9.5 mouse embryos. The size of the SacI fragment in the targeted allele is 8 kb, versus 15 kb in the wild-type allele.