Skip to main content
PMC home page
Journal of Bacteriology logoLink to Journal of Bacteriology
. 1988 Sep;170(9):3978–3982. doi: 10.1128/jb.170.9.3978-3982.1988

Efficiency of homologous DNA recombination varies along the Bacillus subtilis chromosome.

V Vagner 1, S D Ehrlich 1
PMCID: PMC211398  PMID: 3137211

Abstract

Structures consisting of a genetic marker (erythromycin or kanamycin resistance, thymidylate synthetase) flanked by 3.4-kilobase direct repeats (pBR322 sequences) were inserted in 12 different locations of the Bacillus subtilis chromosome. Recombination between the repeats was followed by the loss of the genetic marker. Recombination frequencies found in different locations varied from 1.2 X 10(-5) to 40 X 10(-5) per cell generation. Such differences were highly significant (P less than 0.001).

Full text

PDF
3978

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Colman A., Byers M. J., Primrose S. B., Lyons A. Rapid purification of plasmid DNAs by hydroxyapatite chromatography. Eur J Biochem. 1978 Nov 2;91(1):303–310. doi: 10.1111/j.1432-1033.1978.tb20966.x. [DOI] [PubMed] [Google Scholar]
  2. Dagert M., Ehrlich S. D. Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. 1979 May;6(1):23–28. doi: 10.1016/0378-1119(79)90082-9. [DOI] [PubMed] [Google Scholar]
  3. Edlund T., Grundström T., Normark S. Isolation and characterization of DNA repetitions carrying the chromosomal beta-lactamase gene of Escherichia coli K-12. Mol Gen Genet. 1979 Jun 7;173(2):115–125. doi: 10.1007/BF00330301. [DOI] [PubMed] [Google Scholar]
  4. Harris-Warrick R. M., Elkana Y., Ehrlich S. D., Lederberg J. Electrophoretic separation of Bacillus subtilis genes. Proc Natl Acad Sci U S A. 1975 Jun;72(6):2207–2211. doi: 10.1073/pnas.72.6.2207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Horinouchi S., Weisblum B. Nucleotide sequence and functional map of pE194, a plasmid that specifies inducible resistance to macrolide, lincosamide, and streptogramin type B antibodies. J Bacteriol. 1982 May;150(2):804–814. doi: 10.1128/jb.150.2.804-814.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Jannière L., Ehrlich S. D. Recombination between short repeated sequences is more frequent in plasmids than in the chromosome of Bacillus subtilis. Mol Gen Genet. 1987 Nov;210(1):116–121. doi: 10.1007/BF00337766. [DOI] [PubMed] [Google Scholar]
  7. Jannière L., Niaudet B., Pierre E., Ehrlich S. D. Stable gene amplification in the chromosome of Bacillus subtilis. Gene. 1985;40(1):47–55. doi: 10.1016/0378-1119(85)90023-x. [DOI] [PubMed] [Google Scholar]
  8. Luria S. E., Delbrück M. Mutations of Bacteria from Virus Sensitivity to Virus Resistance. Genetics. 1943 Nov;28(6):491–511. doi: 10.1093/genetics/28.6.491. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. McKenzie T., Hoshino T., Tanaka T., Sueoka N. The nucleotide sequence of pUB110: some salient features in relation to replication and its regulation. Plasmid. 1986 Mar;15(2):93–103. doi: 10.1016/0147-619x(86)90046-6. [DOI] [PubMed] [Google Scholar]
  10. Michel B., Ehrlich S. D. Recombination efficiency is a quadratic function of the length of homology during plasmid transformation of Bacillus subtilis protoplasts and Escherichia coli competent cells. EMBO J. 1984 Dec 1;3(12):2879–2884. doi: 10.1002/j.1460-2075.1984.tb02224.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Niaudet B., Ehrlich S. D. In vitro genetic labeling of Bacillus subtilis cryptic plasmid pHV400. Plasmid. 1979 Jan;2(1):48–58. doi: 10.1016/0147-619x(79)90005-2. [DOI] [PubMed] [Google Scholar]
  12. Niaudet B., Goze A., Ehrlich S. D. Insertional mutagenesis in Bacillus subtilis: mechanism and use in gene cloning. Gene. 1982 Oct;19(3):277–284. doi: 10.1016/0378-1119(82)90017-8. [DOI] [PubMed] [Google Scholar]
  13. Niaudet B., Jannière L., Ehrlich S. D. Integration of linear, heterologous DNA molecules into the Bacillus subtilis chromosome: mechanism and use in induction of predictable rearrangements. J Bacteriol. 1985 Jul;163(1):111–120. doi: 10.1128/jb.163.1.111-120.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Niaudet B., Jannière L., Ehrlich S. D. Recombination between repeated DNA sequences occurs more often in plasmids than in the chromosome of Bacillus subtilis. Mol Gen Genet. 1984;197(1):46–54. doi: 10.1007/BF00327921. [DOI] [PubMed] [Google Scholar]
  15. Noirot P., Petit M. A., Ehrlich S. D. Plasmid replication stimulates DNA recombination in Bacillus subtilis. J Mol Biol. 1987 Jul 5;196(1):39–48. doi: 10.1016/0022-2836(87)90509-2. [DOI] [PubMed] [Google Scholar]
  16. Normark S., Edlund T., Grundström T., Bergström S., Wolf-Watz H. Escherichia coli K-12 mutants hyperproducing chromosomal beta-lactamase by gene repetitions. J Bacteriol. 1977 Dec;132(3):912–922. doi: 10.1128/jb.132.3.912-922.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Rigby P. W., Dieckmann M., Rhodes C., Berg P. Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase I. J Mol Biol. 1977 Jun 15;113(1):237–251. doi: 10.1016/0022-2836(77)90052-3. [DOI] [PubMed] [Google Scholar]
  18. Smith G. R., Amundsen S. K., Chaudhury A. M., Cheng K. C., Ponticelli A. S., Roberts C. M., Schultz D. W., Taylor A. F. Roles of RecBC enzyme and chi sites in homologous recombination. Cold Spring Harb Symp Quant Biol. 1984;49:485–495. doi: 10.1101/sqb.1984.049.01.055. [DOI] [PubMed] [Google Scholar]
  19. Southern E. M. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J Mol Biol. 1975 Nov 5;98(3):503–517. doi: 10.1016/s0022-2836(75)80083-0. [DOI] [PubMed] [Google Scholar]
  20. Sutcliffe J. G. Complete nucleotide sequence of the Escherichia coli plasmid pBR322. Cold Spring Harb Symp Quant Biol. 1979;43(Pt 1):77–90. doi: 10.1101/sqb.1979.043.01.013. [DOI] [PubMed] [Google Scholar]
  21. Wilson M. C., Farmer J. L., Rothman F. Thymidylate synthesis and aminopterin resistance in Bacillus subtilis. J Bacteriol. 1966 Jul;92(1):186–196. doi: 10.1128/jb.92.1.186-196.1966. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Young M. Gene amplification in Bacillus subtilis. J Gen Microbiol. 1984 Jul;130(7):1613–1621. doi: 10.1099/00221287-130-7-1613. [DOI] [PubMed] [Google Scholar]
  23. Youngman P., Perkins J. B., Losick R. A novel method for the rapid cloning in Escherichia coli of Bacillus subtilis chromosomal DNA adjacent to Tn917 insertions. Mol Gen Genet. 1984;195(3):424–433. doi: 10.1007/BF00341443. [DOI] [PubMed] [Google Scholar]
  24. te Riele H., Michel B., Ehrlich S. D. Are single-stranded circles intermediates in plasmid DNA replication? EMBO J. 1986 Mar;5(3):631–637. doi: 10.1002/j.1460-2075.1986.tb04257.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  25. te Riele H., Michel B., Ehrlich S. D. Single-stranded plasmid DNA in Bacillus subtilis and Staphylococcus aureus. Proc Natl Acad Sci U S A. 1986 Apr;83(8):2541–2545. doi: 10.1073/pnas.83.8.2541. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Bacteriology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES