Skip to main content
. 1998 Jul 21;95(15):8703–8708. doi: 10.1073/pnas.95.15.8703

Figure 2.

Figure 2

Expression of ZNF217 and NABC1. (a) Hybridization of a ZNF217 probe to multiple tissue poly(A)+ Northern blots. (b) Northern blot analysis of ZNF217 transcription in three independent karyotypically normal breast epithelial cultures of finite lifespan (161RM, 48RM, 185RM), one immortalized line (184BS) and five breast cancer cell lines (MDA10, SKBR3, MDA436, BT474, MCF7). The breast cancer cell lines carry 4, 10–15, 10–15, >20, and >20 copies of the region encoding ZNF217, respectively. (c) Northern blot analysis of NABC1 transcription in the same cell lines. (d) Analysis of ZNF217 transcription in four paired breast tumor/normal epithelium samples. Lanes containing RNA from normal and tumor tissues are labeled N and T, respectively. (e) Analysis of NABC1 transcription by using the same membrane described in d. (f) QPCR analysis of ZNF217 mRNA abundance relative to that for GAPDH in 11 primary breast tumors and nine breast cancer cell lines. DNA sequence copy number at the ZNF217 locus determined by FISH with RMC20B4097 is indicated above each QPCR measurement.