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A upf3-Δ strain cannot maintain the M1 killer virus. (A) Killer assay of upf mutant strains. Colonies of these strains were grown onto a lawn of cells that are sensitive to the secreted killer toxin produced by the M1 virus. Killer activity was observed as a zone of growth inhibition around the colonies. (B) Total RNAs were isolated from the same strains and analyzed by Northern blotting for the presence of L-A and M1 viral RNAs.
