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. 1998 Jul 21;95(15):8733–8738. doi: 10.1073/pnas.95.15.8733

Figure 2.

Figure 2

Construction of BLM- and WRN-bearing yeast strains. (a) Structures of the sgs1∷BLM+ and sgs1∷WRN+strains. To construct these strains, we inserted a GAPDH promoter-BLM cDNA (or WRN cDNA)-ADH terminator-TRP1 fragment into the SGS1 locus of S. cerevisiae DH6.61D by a one-step gene replacement method (25). The BLM cDNA (or WRN cDNA) was connected with the GAPDH promoter in the correct orientation. Arrows indicate the orientation of GAPDH promoter and other genes. (b) Structures of the sgs1∷BLM− and sgs1∷WRN− strains. The sgs1∷BLM− and sgs1∷WRN− strains are also constructed by insertion into the SGS1 locus as shown above, except that the BLM cDNA (or WRN cDNA) was connected with the GAPDH promoter in the opposite orientation.