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. 1998 Jul 21;95(15):8750–8755. doi: 10.1073/pnas.95.15.8750

Figure 1.

Figure 1

RNase protection analysis of liver steady-state RNAs. (A) Liver RNA (30 μg) from B10aw18 mice (males, lanes 1 and 2, or females, lanes 3 and 4) or BALB/c female mice (lane 6) were hybridized to the transcription start-site probe (spliced and unspliced protected fragments of 121 nt and 234 nt, respectively, are shown schematically). Lanes: 1, 3, and 6, nuclear RNAs; 2 and 4, total RNA; 5, tRNA, negative control. (B) C4-Slp expression in B10aw18 or BALB/c females injected for 20 days with 700 μg of testosterone propionate (+T, lanes 2 and 4). A positive control is in lane 3 (B10aw18 male). Lanes 1 and 5 are untreated controls. Thirty micrograms of total RNA was hybridized to the distal C4/C4-Slp probe (244 nt; described in ref. 2), which distinguishes C4 (100 nt) and C4-Slp (191 nt).