Figure 4.

Time course of liver STAT5 activation upon testosterone treatment. (A) Mobility shifts using liver nuclear extracts from males or from females treated with testosterone for various times. Arrows mark the position of the shifted band in experiments with different times of gel migration. The probe was an oligonucleotide carrying the STAT5 consensus sequence (see Materials and Methods). Exposure times were 15 hr and 48 hr for male and female extracts, respectively. (B) Early detection of activated STAT5 in liver nuclear extracts of androgen-treated females (2 or 4 days). The probe was the same as in A. An arrow points to a weak but significant shifted band observed after 2 days of androgen treatment. Exposure time was 4 days.