Figure 5.
Selective STAT5 binding to upstream GAS sites of C4-Slp. C4-Slp GAS sequences and the corresponding C4 control sequences are shown along the top of the figure, and the most conserved nucleotides of the consensus sequence for STAT5 binding are underlined. Numbers indicate the position of the first T in the C4-Slp sequence. Retardation gel shift assays were performed with probes encompassing GAS 1 and GAS 2 sequences (described in Materials and Methods) and liver nuclear extracts from male (m) or female (f) B10aw18 mice. Competition was performed with 50-fold excess of unlabeled probe in the binding reaction. An arrow indicates the position of the protein–probe complex. Signals of various intensities at the top of each lane correspond to the origin of gel migration. Exposure was for 2 days.