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. 1998 Jul 21;95(15):8756–8760. doi: 10.1073/pnas.95.15.8756

Figure 2.

Figure 2

TPA induction of luciferase mRNA. Total RNA isolated from the stably transfected clonal cell line HS2(2.2)-1, treated with 5 nM TPA for the indicated time, was examined by Northern blot analysis. Shown are the results of successive hybridization of the same filter to the luciferase, γ-globin, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene probes. Signal intensities were quantified by using a PhosphorImager, and luciferase and γ-globin mRNA levels, normalized to those of GAPDH, were plotted.