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. 1998 Jul 21;95(15):8756–8760. doi: 10.1073/pnas.95.15.8756

Figure 3.

Figure 3

NF-E2 sites within HS2 are required for TPA induction. (A) Stably transfected pools of K562 cells containing either the γluc, HS2γluc, HS3γluc, or miniLCRγluc constructs were treated with either 5 nM TPA or vehicle for 20 h and then analyzed for luciferase activity as a measure of γ-globin promoter activity. The fold induction for each pool (mean ± SEM, triplicate measurements) is shown plotted, and the mean activity ± SEM for each construct is shown in the inset. (B) Stably transfected pools containing either HS3γluc (n = 6), HS3(5.1)γluc (n = 3), miniLCRγluc (n = 3), miniLCR(5.1)γluc (n = 4), or miniLCR(7.3)γluc (n = 4) constructs were analyzed for the effect of TPA as above. The mean fold induction ± SEM is shown plotted, and the mean activity ± SEM is shown in the Inset. (C) Stably transfected pools containing either ΔNF-E2 (n = 10), ΔHS2NF5 (n = 4), ΔUSF (n = 4), or HS2γluc (n = 8) constructs were analyzed as above. The mean fold induction ± SEM is shown plotted, and the mean activity ± SEM is shown in the Inset. At least triplicate measurements were made for each sample.