Table 1.

NF-E2 is required for TPA induction

Cells	DMSO treatment	Average luc activity, RLU/s per μg		Average fold induction
		−TPA	+TPA
CB3	No	440  ± 240	450  ± 240	1.04  ± 0.02
CB3 + NF-E2	No	4,580  ± 1,580	6,780  ± 2,840	1.41  ± 0.12
CB3	Yes	700  ± 290	690  ± 300	0.98  ± 0.04
CB3 + NF-E2	Yes	7,400  ± 1,900	10,000  ± 3,400	1.30  ± 0.13

Stably transfected pools of CB3 cells containing either HS2βluc (n = 3) or HS2βluc and the NF-E2 expression vector pEF1αneo45-G1 (n = 3) were treated with either 5 nM TPA or vehicle for 20 h, both in the presence and absence of 1.8% dimethyl sulfoxide (DMSO), and then analyzed for luciferase activity as a measure of β-globin promoter activity (mean ± SEM). At least triplicate measurements were made for each sample.