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Afr1 regulation of Δ10 transgene expression. RNA was isolated from livers of 4-week-old transgenic mice from BALB/cJ matings. (A) Typical results of RNase protection assays using 100 μg of total RNA. (B) Transgenic mice were grouped by Afr1 phenotype as determined by the level of endogenous AFP gene expression and the level of AFP MG mRNA plotted. (Δ10A: Ab, n = 19; bb n = 10; Δ10B: Ab, n = 21; bb n = 17.)
