Figure 1.

Denaturing gradient gel analysis of polyclonal (PBLs) and clonal TCR transcripts covering BV5, BV8, and BV12. cDNA from PBLs was amplified with primers specific for BV families 5, 8, or 12, cloned into PCR (Invitrogen), and reamplified. PCR products were loaded onto a 20–80% denaturing gradient gel and run for 4.5 hr at 160 V at a constant temperature of 58°C. DNA was stained with ethidium bromide and photographed under UV light.