Skip to main content
. 1998 Jul 21;95(15):8785–8790. doi: 10.1073/pnas.95.15.8785

Figure 3.

Figure 3

Denaturing gradient gel analysis for clonality in tumor-infiltrating lymphocytes in B16 G3.12, B78–D14/PBS, and B78–D14 ch14.18–IL-2. From four animals in each group (left to right) cDNA from tumor biopsies were PCR amplified with primers specific for BV5, BV8, and BV12 along with the GC-clamped constant regions primer. Aliquots were loaded onto a 20–80% denaturing gradient gel and run for 4.5 hr at 160 V at a constant temperature of 58°C. DNA was stained with ethidium bromide and photographed under UV light.