Table 2.
Relative expression of TCRBV regions 1-18 in B16 G3.12 and B78–D14 tumors in PBS-treated animals as well as B78–D14 tumors in ch14.18–IL-2-treated animals
| B16/7 days of PBS
|
B16/DG2/7 days of PBS
|
B16/DG2/7 days of ch14.18–IL-2
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Animal 1 | Animal 2 | Animal 3 | Animal 4 | Animal 1 | Animal 2 | Animal 3 | Animal 4 | Animal 1 | Animal 2 | Animal 3 | Animal 4 | |
| % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | % (SD) | |
| BV1 | 5.4 (0.4) | 4.9 (1.4) | 11.3 (ND) | 5.2 (0.7) | 10.9 (0.7) | 2.8 (1.5) | 9.4 (5.2) | 4.3 (0.9) | 6.1 (0.1) | 3.4 (0.2) | 4.4 (0.4) | 6.8 (2.2) |
| BV2 | 4.2 (0.9) | 5.1 (1.8) | 4.1 (ND) | 5.9 (1.8) | 5.4 (0.7) | 7.2 (1.9) | 4.4 (1.7) | 6.1 (0.3) | 5.4 (0.6) | 6.5 (1.1) | 4.7 (0.7) | 7.1 (0.4) |
| BV3 | 5.7 (0.5) | 4.9 (1.4) | 1.2 (ND) | 2.7 (1.1) | 2.9 (0.2) | 3.6 (1.0) | 4.0 (0.6) | 2.6 (0.3) | 2.5 (0.6) | 1.4 (0.6) | 2.7 (0.5) | 4.5 (0.9) |
| BV4 | 1.6 (0.6) | 4.2 (0.1) | 2.5 (ND) | 2.1 (0.5) | 3.4 (0.0) | 6.8 (3.2) | 2.2 (0.2) | 3.3 (0.3) | 2.1 (0.6) | 1.4 (0.1) | 2.2 (0.1) | 2.7 (1.3) |
| BV5 | 8.1 (0.6) | 6.2 (0.3) | 5.9 (ND) | 7.7 (2.3) | 4.7 (0.4) | 6.2 (1.3) | 5.2 (0.6) | 6.3 (1.7) | 11.6 (2.4) | 28.8 (1.2) | 17.5 (0.6) | 10.3 (1.4) |
| BV6 | 12.7 (0.4) | 6.4 (1.3) | 19.9 (ND) | 5.0 (0.1) | 5.2 (1.7) | 3.9 (0.4) | 5.9 (0.8) | 5.4 (0.8) | 6.9 (0.4) | 5.2 (0.8) | 4.1 (0.1) | 5.1 (0.3) |
| BV7 | 3.7 (1.2) | 4.3 (0.6) | 2.3 (ND) | 3.5 (1.9) | 2.9 (0.8) | 2.8 (0.8) | 3.4 (0.8) | 3.6 (0.3) | 6.0 (1.1) | 4.6 (1.1) | 6.8 (1.4) | 5.8 (0.2) |
| BV8 | 13.2 (0.2) | 5.3 (0.2) | 10.5 (ND) | 18.9 (1.7) | 30.4 (4.0) | 10.1 (0.7) | 22.7 (1.1) | 14.9 (1.4) | 18.2 (0.4) | 16.8 (0.5) | 17.7 (0.7) | 9.3 (3.1) |
| BV9 | 4.0 (1.2) | 4.3 (1.3) | 1.6 (ND) | 3.7 (1.1) | 4.0 (0.7) | 3.0 (0.7) | 6.0 (0.6) | 3.7 (0.1) | 5.8 (0.1) | 3.8 (0.1) | 3.3 (1.2) | 5.2 (0.4) |
| BV10 | 3.1 (0.8) | 7.4 (0.3) | 3.1 (ND) | 4.8 (0.3) | 4.0 (0.1) | 6.1 (0.3) | 6.0 (1.0) | 6.2 (0.6) | 5.4 (1.1) | 4.8 (1.3) | 7.8 (0.5) | 6.1 (0.6) |
| BV11 | 11.5 (2.6) | 5.7 (0.1) | 12.6 (ND) | 9.0 (3.1) | 7.1 (1.2) | 5.4 (0.6) | 2.5 (0.4) | 5.4 (0.2) | 5.2 (0.9) | 6.1 (0.1) | 4.7 (0.8) | 5.3 (1.8) |
| BV12 | 5.8 (0.7) | 8.4 (1.3) | 10.8 (ND) | 6.2 (0.0) | 3.5 (1.3) | 12.9 (0.5) | 5.9 (1.0) | 15.5 (0.0) | 7.5 (1.9) | 6.7 (1.6) | 10.5 (0.6) | 7.7 (1.1) |
| BV13 | 9.9 (4.0) | 10.2 (1.4) | 3.4 (ND) | 5.1 (1.2) | 2.3 (0.3) | 4.4 (0.5) | 3.3 (0.0) | 4.7 (0.3) | 6.5 (1.5) | 3.8 (1.3) | 4.1 (0.8) | 6.8 (0.2) |
| BV14 | 2.8 (1.3) | 7.5 (2.1) | 2.4 (ND) | 3.0 (0.1) | 2.5 (0.5) | 9.8 (0.2) | 4.5 (0.7) | 3.8 (1.0) | 2.5 (0.0) | 2.3 (0.5) | 3.2 (0.3) | 5.7 (0.4) |
| BV15 | 3.8 (1.0) | 5.0 (1.0) | 2.2 (ND) | 4.5 (0.2) | 5.0 (0.1) | 6.6 (0.1) | 9.3 (0.4) | 6.2 (0.0) | 2.0 (0.0) | 1.4 (0.5) | 2.1 (0.3) | 4.5 (0.3) |
| BV16 | 3.9 (0.2) | 5.9 (1.8) | 4.6 (ND) | 6.4 (0.8) | 5.0 (0.2) | 7.2 (1.0) | 4.6 (0.9) | 7.3 (2.3) | 5.1 (0.1) | 2.8 (0.2) | 3.6 (0.4) | 4.5 (0.3) |
| BV17 | 0.2 (0.1) | 1.1 (0.9) | 0.7 (ND) | 0.4 (0.2) | 0.1 (0.0) | 0.3 (0.0) | 0.4 (0.1) | 0.1 (0.0) | 0.5 (0.1) | 0.3 (0.1) | 0.4 (0.4) | 0.2 (0.0) |
| BV18 | 0.4 (0.2) | 3.3 (0.0) | 0.5 (ND) | 5.7 (0.9) | 0.7 (0.1) | 0.9 (0.7) | 0.1 (0.1) | 0.4 (0.0) | 0.7 (0.4) | 0.2 (0.1) | 0.2 (0.1) | 0.4 (0.1) |
The expression of each BV region was calculated as a percentage of the sum of all BV spots.