Figure 1.

Transient transfection of HT-1080 cells with sflt-1 cDNA inhibits lung implantation and growth. Human HT-1080 fibrosarcoma cells were transiently transfected with plasmid expressing sFLT-1 or the corresponding control plasmid and 8 × 10⁵ cells in 0.3 ml were injected via the tail vein (n = 9/group). After 30 days lungs were removed and examined histologically to detect tumor nodules, defined by clusters containing ≥6 tumor cells. The statistical difference between groups was computed using χ² statistics (∗, P < 0.02).