Figure 5.
Survival kinetics of SCID mice injected intracranially with D54-MG human glioma cells stably transfected with sflt-1 cDNA. D54-MG human glioma cells (5 × 105) either not transfected (open squares) or stably transfected with either pcDNA3 (stippled squares) or psflt-1 (filled circles) were stereotactically injected into brains of SCID mice (n = 5/group) to a depth of 2.5 mm after which they were monitored twice daily. Survival of animals injected with tumor cells expressing the sflt-1 gene was significantly (P < 0.005; Kaplan–Meier survival analysis, Mantel–Cox log rank test) longer than those injected with tumor cells transfected with either control pcDNA3 or no plasmid which were not different from each other.