Table 1.
Apoptosis in nontransfected and bcl-2-transfected MCF7 cells
| Ligands | Apoptotic cells, %
|
||
|---|---|---|---|
| non-transfected | bcl-2 expression vector | empty vector | |
| (−) | 8 ± 3 | 10 ± 3 | 12 ± 4 |
| ATRA | 7 ± 5 | 11 ± 5 | 14 ± 6 |
| TGZ | 13 ± 8 | 11 ± 6 | 20 ± 9 |
| TGZ + ATRA | 41 ± 4 | 10 ± 4 | 58 ± 8 |
| IND | 12 ± 4 | ND | ND |
| IND + ATRA | 44 ± 6 | ND | ND |
| 15dPGJ2 | 18 ± 7 | ND | ND |
| 15dPGJ2 + ATRA | 46 ± 4 | ND | ND |
Apoptosis determined by TUNEL assay. ND, not done; IND, indomethacin. Cells exposed 4 days to ATRA (10−6 M), TGZ (10−5 M), IND (10−5 M), and 15dPGJ2 (10−5 M). Results represent the mean ± SD of three experiments.