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. 1998 Jul 21;95(15):8817–8822. doi: 10.1073/pnas.95.15.8817

Figure 6.

Figure 6

In vitro translation of VHL gene products. In vitro transcription/translation was performed in the presence of [35S]methionine. Heterologous leader sequence plasmids VHLp24(MPR), VHLp18(MEA), and pCR3 and native 5′ untranslated sequence constructs VHLNT, VHLNT(M54I), and VHLNT(M1I) were used as templates (as indicated above the lanes). Fifteen microliters of the resulting products was immunoprecipitated with mAb 11E12, separated on an SDS/13.5% polyacrylamide gel, and visualized by fluorography. Positions of the VHLp24(MPR) and VHLp18(MEA) products are indicated to the left.