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C. parvum treatment induces wt p53 accumulation in mouse liver. Liver protein extracts of wt p53 mice were prepared 10 and 28 days after injection of C. parvum (100 mg/kg); tissue extracts of two animals per group were analyzed. p53 protein was immunoprecipitated by using 300 μg of protein extract and 10 μl of a polyclonal p53 antiserum (CM-1) and was detected with the monoclonal antibody PAb 246. p53 is highly expressed 10 and 28 days after the treatment with C. parvum.
