Figure 2.

AdExTek delivers ExTek serum levels in excess of levels required to block Ang binding and Ang-stimulated cellular responses. (A) ExTek expression from cultured 293 cells infected with AdExTek virus. Three days after AdExTek infection, 25 μl of culture media was separated by SDS/PAGE, transferred to nitrocellulose, and immunoblotted with anti-Tie2 Ab33 (lane 1). Purified ExTek.6His protein (100 ng) was used as a control (lane 2). (B) Plasma ExTek concentrations after systemic administration of AdExTek. AdExTek (5 × 10⁸ pfu) was administered to mice i.v. ExTek concentration in plasma was determined by a simple ELISA as described in Methods. Data are plotted as the mean ± SE of five mice. For some points error bars are smaller than the symbols.