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RT-PCR analysis of human IGF-II (a) and β-actin (b) mRNA expression in DU-145 prostate tumors. PCR products were separated by 1.8% agarose gel electrophoresis and stained with ethidium bromide. The sizes of expected PCR products were 538 and 518 bp for hIGF-II and hβ-actin, respectively. Lanes: M (molecular weight marker), MX174 HaeIII digest; 1, negative control; 2–6, samples from untreated animals; 7–11, tumor samples from animals treated with GH-RH antagonist MZ-5-156.
